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KMID : 1123920170310020111
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Korean Journal of Oriental Physiology and Pathology
2017 Volume.31 No. 2 p.111 ~ p.117
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Synergistic Anticancer Effect of Astragalus Membranaceus and Adenophora Triphylla Var. Japonica in H1299 Human Lung Carcinoma Cells
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Lee Ji-MIn
Kim Hong-Jae
Choi Yung-Hyun
Chi Gyoo-Yong
Park Shin-Hyung
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Abstract
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The anti-cancer effects of Astragalus membranaceus (AM) and Adenophora triphylla var. japonica (AT) have been described. Each of their effects mainly focused on the immunopotentiating and apoptosis inducing-ability in several cancer cell lines. Although the combination of AM and AT is occasionally used in Chinese medicine to treat lung cancers, their synergistic effect has not been proved yet. This study was designed to verify whether AM combined with AT exhibits a synergistic anti-cancer effect in H1299 human lung carcinoma cells. The ethanol extracts of AM (EAM) and AT (EAT) showed only slight cytotoxicity in H1299 cells when treated alone. However, the combination of EAM and EAT markedly suppressed the cell growth measured by MTT assay and trypan blue counting assay. In addition, co-treatment of EAM with EAT significantly reduced the colony-forming ability compared with single treatment of EAM or EAT in H1299 cells. We demonstrated that the synergistic effect of AM and AT was related with apoptosis induction proved by an accumulation of chromatin condensation, annexin V-positive cells, sub-G1 phase population, and cleaved-PARP expression, which were not observed by single treatment of EAM or EAT. In conclusion, the combination of EAM and EAT exhibited superior anti-cancer activity in H1299 cells than single treatment of EAM or EAT. We suggest that EAM combined with EAT might be a novel therapeutic option for lung cancer patients, and provide a reference for the development of more effective combination of Chinese herbs to treat lung cancer.
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KEYWORD
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Astragalus membranaceus, Adenophora triphylla var. japonica, Lung cancer, Apoptosis, Combination therapy
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